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Update on My Ideas for Elm Phloem Necrosis

Previously I proposed three possible solutions for Elm Phloem Necrosis: Developing resistant Elm cultivars, a membrane that would zap the bacteria as it passes through, and silver nanoparticles that would rip the bacteria’s membrane. I proposed the silver nanoparticles was the quickest solution that could be implemented today. I have learned a few things since then.

It turns out I am not the only one who has thought of using silver nanoparticles to treat Elm Phloem Necrosis. Dr. Moorman from Penn State also thought of this and tried a treatment. He was unable to see if it was successful all the Elm trees died from Elm Phloem Necrosis or Dutch Elm Disease. He suggested to me that Elm trees have a bigger problem which is disease susceptibility. If the Elm tree does not die from Elm Yellows, Dutch Elm Disease may kill it. We have solutions for Dutch Elm Disease however they are expensive and need to be applied each year or each time it’s infected. This means that the development of resistant Elm cultivars is the long term solution for Elm trees.

How can we do this? Genetic Engineering. We can either breed or use modern molecular biology techniques to create a new Elm Cultivar. Unfortunately, breeding takes decades to develop a new cultivar and by that time Elm trees will likely be gone from North America.

How can we develop a new Elm cultivar with biotechnology? Using TransChromo insertion, meaning inserting a whole chromosome, or large piece of DNA into another organism. One tool we can use to insert a gene into an organism is a gene gun. It does what it sounds like it does. Although a better name would be gold gene gun. The gene gun shoots gold particles that are coated with a target gene into a cell of the organism. The problem with this technique is that the gene will be expressed for a while, but after a while, the expression of the gene will fade and may completely be silenced. We have improved the method by inserting the target gene with regulation genes with it to tell the cell to express the gene we want. There is still a problem which is that with the gene gun there is no control on where the gene(s) are inserted. And where it is inserted can play a huge role on how the gene is expressed. My solution is to insert a larger piece of DNA instead of one gene, a much larger piece, as large as we can feasibly insert with the gene gun.

To do this we would extract DNA from the organism with the target gene. The gene would then be marked using a genetic marker. This genome would then be cut up using restriction enzymes. The marked DNA fragment with the target gene would then amplified using polymerase chain reaction (PCR). The amplified DNA with the target gene is painted onto the gold particles and shot into the organism we want the express the target gene.

What is the advantage of this method? It would provide a better way to have long term expression of the target gene and provide more genetic variability for the TransChromo organism from the other genetic information that was inserted. What about the Elm trees?

Oh yeah, the Elms. This method could be used to insert large pieces of DNA from relatives of the Elm such as members of the genus Ampelocera and the genus Zelkova. Both may have defense genes that provide resistance to the diseases and pests affecting Elms today.

Eliminating the pests of Elm trees is unfeasible because Elm trees lack genetic information for the defensive of these pests. A better solution is to develop a resistant Elm cultivar to current Elm diseases and pests. A way to do this is TransChromo insertion, using a gene gun to insert a large piece of DNA into an organism. This method may provide the resistance needed for the long term viability for Elm trees.

 

Three elm leaves that are yellow and dying. The elm leaves have holes on the edges. There is a black or dark brown color on the edges as well.

Elliott Killian

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